The Gophers' undefeated season is over after losing to Iowa 23-19.
The Gophers dropped their third consecutive game.
"We've done a lot of things we haven't done in a while," said senior Carter Coughlin. "We're going to keep that same mentality, and trust me when I say this week, we are going to practice harder than we've ever practiced. We've got our eyes fixed on the goal, which is going 1-0 in the Iowa season."
Minnesota's offense struggled against Butler, shooting 33 percent from the field.
Missed opportunities cost Minnesota as they surrendered a second-half lead.
"Just seeing the stadium and how much it meant to the fans was really cool," said quarterback Tanner Morgan. "When they rushed the field, that's a moment I'll never forget."
In 2013, University of Minnesota alumnus Ben Elliott donated bone marrow to Doug Parisano, a Pennsylvania resident. On Saturday, the two will reunite at TCF Bank Stadium Saturday as the Gophers take on Penn State.
The Gophers' new dynamic back court powered them to a 85-50 victory over Cleveland State in their season opener.
The University of Minnesota announced a contract extension with football head coach P.J. Fleck Tuesday, pending approval from the Board of Regents.